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Then, the cells were stained with 4,6-diamidino-2-phenylindole (DAPI) and analysed with BD FACSAria TM III (BD Biosciences)

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Li et al demonstrated that vitamin B12 may up-regulated insulin like growth factor-1 (IGF-1) gene expression, and showed a better neuroprotective effect in the presence of good control of hyperglycemia in rats with diabetic peripheral neuropathy 20